Studies on Cryotop Vitrification Method for Simple Freezing of Hanwoo Embryos

A Simple Method for Freezing Bovine Embryos

Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method

Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow...

Mice Embryos Cryopreservation: Vitrification or Ultra-Rapid Freezing?

Background: The importance of mice as animal model for research has promoted the surge of many strains with important characteristics which need to be preserved. Embryo cryopreservation appears as the most suitable technique. However, until now there is not an effective methodology for this specie. This study aimed to evaluate three methods for mice embryos cryopreservation. Materials, Methods ...

The effect of vitrification on mouse oocyte apoptosis by cryotop method.

BACKGROUND Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. METHOD A total of 200 germinal vesicle (...

The Effect of Vitrification on Mouse Oocytes Apoptosis by Cryotop Method

Background: Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. Method: A total of 200 germinal vesicle (...